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Abberior Instruments STED Superresolution

Abberior Instruments GmbH was founded in 2012 by Dr. Gerald Donnert, Dr. Alexander Egner, Dr. Benjamin Harke, Prof. Dr. Dr. h.c. Stefan W. Hell, Dr. Lars Kastrup, Dr. Matthias Reuss and Dr. Andreas Schönle. The company is a spin-off from the Max Planck Institute of Biophysical Chemistry in Göttingen and the German Cancer Research Institute in Heidelberg.

1. STEDYCON
Abberior Instruments is known for extraordinary customization ability of its flexible STED microscope platform. Abberior has developed the ultimate compact STED and confocal microscope, called STEDYCON.

STEDYCON is a completely new class of nanoscope. It converts your conventional epifluorescence microscope into a versatile four-color confocal (405nm, 488nm, 561nm, 640nm) and STED (775nm) system! At the same time, it is incredibly compact and can be used by anyone!

• Convert your existing microscope instantly
• Up to four detectors with superior
DynamicPLUS Imaging
• New STEADYFOCUS module to eliminate
focus drift
• Super-stable
• Top-notch STED performance
• Plug & Play
• Save Space
• Service and maintenance free



Hela cells with actin cytoskeleton labelled (Phalloidin, Abberior STAR RED). Imaged with Abberior Instruments’ STEDYCON and deconvolved with SVI Huygens optimized for STEDYCON.

Examples of multicolour live-cell time-lapse confocal imaging using the STEDYFOCUS over many hours.


2. Expert Line

The Expert Line is our Flagship product: a sophisticated, highly flexible open platform that can be extended and upgraded, even after purchase.


  • Multicolor confocal scanning system with variable pinhole size
  • Detector gating
  • Accessible optomechanical design, open electronics and software platform, ready to implement your own imaging ideas
  • Zero-compromize on performance - get outstanding resolution performance in STED microscopy
  • QUAD beam scanner technology
  • We offer unrivaled flexibility in customizing the microscope to your applications
  • We install your turn-key nanoscope in your lab including software/handling training
3-color RESCue STED images using both pulsed STED lasers @775nm and @595nm. Labelled structures: Nuclear pore complex (green, nup153, Oregon Green 488), Vimentin (white, Abberior STAR 635P) and lamin (red, Abberior STAR 580).

Nuclear pore complex protein (nup153). Resolution (STED): < 25 nm.

3-color STED image of primary hippocampal neurons. Please note the characteristic ~190 nm beta II spectrin periodicity along distal axons (green) which is only visible in the STED image. Labelled structures: beta II Spectrin (green, Abberior STAR635P), Bassoon (red, Abberior STAR580), Actin cytoskeleton (blue, Phalloidin, Oregon Green 488). Imaged with Abberior Expert Line with 595nm and 775nm STED laser. Sample was prepared by Elisa D’Este @ MPIBPC, Göttingen

3D superresolution volume image of active zones at the Drosophila larval neuromuscular junction. Immunostaining for Bruchpilot and a second protein. Recorded using easy3D STED at 775nm. Samples by M. Lenz & M. Landgraf (University of Cambridge, UK).

3. Facility Line

The Facility Line is a truely cutting-edge microscope that is incredibly easy to use. The Facility Line combines advanced features, such as high-end confocal and superresolution imaging, Adaptive Illumination (DyMIN, RESCue, MINFIELD), Adaptive Optics, easy3D STED, confocal & STED autofocus, spectral Rainbow Detection, and full autoalignment.

Impressions
Abberior STAR dye triple-stained mammalian cell. Vimentin is highlighted with the brand new Abberior STAR GREEN (green), nuclear pores stained with Abberior STAR 580 (red) and peroxisomes are labeled with Abberior STAR RED (gray).

Primary hippocampal neurons with cytoskeleton proteins labeled (magenta, alpha-Adducin, Abberior STAR 635P and green, ßII spectrin, Alexa 594).

Videos

Confocal and easy3D DyMIN STED imaging of dendrites in brain slices. GFP-tagged proteins were expressed and immunolabelled using primary antibodies against GFP and secondary antibodies coupled to Star635P. Sample was prepared by O. Kaplan and H. Kawabe @ MPI of Experimental Medicine, Göttingen, Germany. Scale Bar, 2 µm.

Contact Us
If you would like to obtain more info and learn more of its applications, please contact us.